Aqueous Liquid To Liquid Transfers With Uncoated Pins

 
   

PIN

DIAMETER

SHAPE

DELIVERY VOLUME RANGE*#

FP9 0.229 mm solid 13 - 39 nl
FP8 0.356 mm solid 15 - 37 nl
FP1 0.457 mm solid 22 - 61 nl
FP1S6 0.457 mm 6 nl slot 34 - 67 nl
FP1S10 0.457 mm 10 nl slot 39 - 74 nl
FP1S20 0.457 mm 20 nl slot
FP1S30 0.457 mm 30 nl slot
FP1S40 0.457 mm 40 nl slot
FP1S50 0.457 mm 50 nl slot 90 - 124 nl
FP3 0.787 mm solid 93 - 213 nl
FP4 0.914 mm solid 126 - 289 nl
FP 1.58 mm solid pointed 175 - 594 nl
FP3S100 0.787 mm 100 nl  slot 213 - 334 nl
FP3S200 0.787 mm 200 nl slot 311 - 449 nl
FP6 1.58 mm solid flat 465 - 960 nl
FP3S500 0.757 mm 500 nl slot 515 - 671 nl
FPS.5 1.58 mm 0.5 ul slot 524 - 962 nl
FP6S.5 1.58 mm 0.5 ul slot 934 -1,445 nl
FPS 1.58 mm 1 ul slot 1,056 - 1,476 nl
FP6S 1.58 mm 1 ul slot 1,396 - 1,930 nl
FPS2 1.58 mm 2 ul slot 1,739 - 2,174 nl
FP6S2 1.58 mm 2 ul slot 2,072 - 2,637 nl
FPS5 1.58 mm 5 ul slot 5,150 - 4,953 nl
FP6S5 1.58 mm 5 ul slot 4,820 - 4,693 nl

 

 

*# delivery volume range is determined by speed of withdrawal from source liquid.
 slow speed  = 0.78 cm/sec = low volume delivery range
 fast speed  =  5.70 cm/sec = high volume delivery range
 Follow this link to assay methods

 

*Speed of withdrawal determines volume transferred. The faster the withdrawal speed the larger the volume transferred.  Withdrawal speed range is from 1.5 mm/sec to 30mm/sec.

 

*The volume of liquid in the source well also makes a significant difference in the volume transferred and there is a difference between sold and slotted pins.  The volume used for the 96 well data was 200 ul and the volume used for the 384 well data was 75 ul.  If you use more or less volume in your wells it will affect the volume transferred.  See this link for more information or See this link for more data.

 

 
         

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