V&P Scientific Seed Loaders are used by plant geneticists to simplify the task of placing tiny seeds, such as Arabidopsis, Rice, Wheat, Canola, or Basil seeds into microplates. By designing the loading holes with precision shape, diameter, depth, and lubricity, the seeds fall easily into holes when the seeds are swirled on the loading plate or “swept” across the surface with a brush. As described in Dr. Patrick Krysan’s article “A High-Throughput Method for Capturing Plant Tissue Samples for Genotype Analysis”, a 97% success rate was achieved using V&P’s Arabadopsis Seed Loader. The Seed Loader’s exact measurements allow only one seed per hole, and our new and improved proprietary design ensures the seeds are deposited by spring loaded pins into the wells of the microplate.
Seed Loaders are very easy to use. Simply place an excess amount of seeds on the seed loader and either swirl the seeds around until they fall into the loading holes or use a brush to sweep across the surface. Once all the loading holes are filled, the excess seeds are removed. Next, place an empty microplate over the seed loader and flip upside-down and spring-loaded pins will physically push the seeds into each well.
V&P can design a variety of custom Seed Loaders for unique applications. We can design a loader to accommodate specific seeds or to load a certain number of seeds per well. The Arabadopsis Seed Loader is the most commonly used Seed Loader due to the seed’s broad application, flexibility, and rapid growth rate.
The application of the prototype Arabidopsis Seed Loader is described by Dr. Patrick Krysan in an article describing "Ice-Cap". A High-Throughput Method for Capturing Plant Tissue Samples for Genotype Analysis.