volume range is determined by speed of withdrawal from source liquid.
slow speed = 0.78 cm/sec = low volume delivery
fast speed = 5.70 cm/sec = high volume delivery range
this link to assay method
*Speed of withdrawal determines volume transferred. The
faster the withdrawal speed the larger the volume
transferred. Withdrawal speed range is from 1.5 mm/sec to
*The volume of liquid in the source well also makes a significant difference in
the volume transferred and there is a difference between sold and slotted pins.
The volume used for the 96 well data was 200 ul and the volume used for the 384
well data was 75 ul. If you use more or less volume in your wells it will
affect the volume transferred. See this link for more information or
See this link for more data.