A Cost-Effective Way To Pipet 384 Samples Simultaneously

 


  John Herich Manager-HTS Maxim Pharmaceutical

     
 

Presented at the San Diego Chapter of the LRIG 9/26/2001

     
 


Maxim HTS Introduction
 

     
 
  • Screen for inhibitors or inducers of apoptosis

  • Proprietary substrate specific for activated caspase

  • Screens are performed in 384 well format

  • Screens are cell based, prefer < 1% DMSO

     
 


Current HTS Equipment
 

     
 
  • Pipetting

    • 96 channel (CyBi Well/Multimek)

    • 4 channel (Multiprobe)

     

  • Plate Movers

    • 384 wells (Specrafluor Plus)

     

  • Plate Movers

    • Robotic Arm (Platecrane)

    • Stackers (Platestak)

     
 


History

 

Upgrade Path to Simultaneous 384 Pipetting

 

Disposable Tip Costs

 

384 Pin Tool Alternative

 

Pin Tool Methods

 

HTS Method With Disposable Tips

 

Pin Tool Method

 

Test Method

 

Just Before Pipetting

 

Dispense

 

Reset Pins

 

Results By Plate

 

Results for a 10 Plate Run

 

Accuracy

 

Conclusions

 

Practical Methods

 

Acknowledgements

 
     
         

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