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Even 2 ml micro centrifuge tubes can be used in these stands.
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Even 2 ml micro centrifuge tubes can be used in these stands. |
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This magnetic bead separation system for 50 ml conical test tubes consists of 2 parts. The VP 772F1M is a magnetic base which set on a base with the same dimensions as a microplate (127.76mm by 85.48 mm) holds four 48 MGO NdFeB magnets that are 25.4 mm cubes. The VP 772F1R is a rack holding eight 50 ml conical test tubes which can be manually or robotically placed over the magnetic base and the magnetic beads in the test tubes are rapidly separated by the large and power magnetic field generated by the VP 772F1M. The photo on the right illustrates the two parts mated together and the photo below it illustrates how they perfectly fit together and hold the test tubes close to the magnets for rapid separation of the beads. Note that we have machined slots in the tube rack so you can see the tubes and when the beads have been separated. The key to our success is that we use
very large magnets with high energy 48 MGO or higher.
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Download video of VP 772F1M
& VP 772F1R in action
The photo to right illustrates a tube with suspended Magnasil beads (Promega) and the photo just below it was taken just 20 seconds later. The same test performed on a similar Promega separation system using much thinner and weaker magnets required twice the time to separate the beads. |
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Single magnetic separation stand made from HDPE (High
Density Poly Ethylene 63.5mm diameter and 61mm tall) for
50ml centrifuge tubes with one magnetic cube (25.4mm on a
side). This very large magnetic cube is made from a very
high energy rare earth magnet (48 MGO NdFeB). The design of
the VP 772FB-1 was constructed to reduce the distance
between the magnet and the tube to zero, thus maximizing the
holding power of the magnet to the beads. This magnet
produces a very large and very powerful magnetic field which
will capture your magnetic beads in just a few seconds and
will tightly hold the beads to the tube wall through the
most rigorous washing procedure.
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| The VP 772FB-1 separation stands can be linked together via the magnetic force of the magnets to form a row of separation stands of any length. |
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Depending on your assay and the liquid volume of your assay,
you may want the location of the magnetically captured beads
in the tube to be lowered. This may be important if you are
using low volumes of incubation or wash buffers. You can
lower the capture level by simply placing quarters in the
bottom of the separation stand and raise the tube up. The
more quarters you put in, the lower the magnetically
captured beads will be held in the tube.
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The VP 772FB-1A is a clear Acrylic cylinder 29.22mm in
diameter and 51.82mm long with a 16.44mm diameter hole
drilled so it is tangent to the magnet edge and has a
16.38mm wide opening to the magnet. The VP 772FB-1A was
designed as an adaptor to fit inside a VP 772FB-1 and allows
the use of test tubes that have diameter smaller than 16.44
mm (15 ml conical centrifuge tubes, 13 ml standard plastic
test tubes and 10 ml glass screw cap test tubes). This
greatly increases the versatility of the VP 772FB-1 magnetic
separation stand. The very large magnetic cube of the VP 772FB-1 is made from a very high energy rare earth magnet (48 MGO NdFeB). The design of the VP 772FB-1A was constructed to reduce the distance between the magnet and the tube to zero, thus maximizing the holding power of the magnet to the beads. This magnet produces a very large and very powerful magnetic field which will capture your magnetic beads in just a few seconds and will tightly hold the beads to the tube wall through the most rigorous washing procedure. If speed of separation and vigorous washing are important in your application this is the magnetic separation stand for you. |
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Top View of the VP 772FB-1A is seen in the photo to the right. Note how the eccentric placement of the hole through the Acrylic rod places the tube against the magnet. An added benefit of the Acrylic cylinder is that it functions as a magnification lens to help visualize the beads. |
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Depending on your assay and the liquid volume of your assay, you may want the height of the magnetically captured beads in the tube to be lowered. You can do this by simply placing quarters in the bottom of the Separation Stand under the VP 772FB-1A. The more quarters you put in, the lower the magnetically captured beads will be held in the tube. This may be important if you are using low volumes of incubation or wash buffers. |
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A 15 ml conical centrifuge tube is shown with a VP 772FB-1 and a VP 772FB-1A and a stack of quarters in the photo to the right. |
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| A 13 ml test tube is shown with a VP 772FB-1 and a VP 772FB-1A and a stack of quarters in the photo to the right. |
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The VP 772F is designed with two 42 MGO NdFeB magnets (51 x 51 x 79 mm) in an aluminum and polycarbonate frame to pellet Magnetic Beads to the sides of four 100 ml glass or 250 ml Nalgene bottles. These large magnets provide a very large and powerful magnetic field that will separate the magnetic beads in |
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Dimensions and specifications for the VP 772F can be found on this page. The key to our success is that we use very large magnets with high energy 48 MGO or higher. |
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This magnetic bead separation system for 250 & 500 ml bottles consists of a powerful 48 MGO NdFeB magnet that is a 25.4 mm cube. The VP 772FA hold 1 bottle at a time and was designed for manual use. The photo to right illustrates a bottle with suspended Magnasil beads (Promega) and the photo just below it was taken just 30 seconds later. The key to our success is that we use very large magnets with high energy 48 MGO or higher.
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The magnetic bead separation system for 1L bottles is designed for easy removal of the cleared supernatant, which can simply be poured off by removing the cap while the bottle is still held securely in the device. A massive 2"x2"x3" Neodymium Iron Boron magnet removes the beads in 5 to 10 minutes and holds them securely while solution is added or removed. This design allows for the use of large volumes of magnetic bead solutions while facilitating rapid washing and resuspension of the beads. |
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The VP 772F2M-1 is a magnetic base with a 48 MGO Neodymium Iron Boron (NdFeB) magnet which works with any of the tube racks.
Magnetic Bead Separator Tube Racks VP 772F2R-1, VP 772F2R-2, VP 772F2R-3 work with 1.5ml tubes (13mm OD), 15ml tubes (16.7mm OD), 50ml tubes (29mm OD) respectively.
The VP 772F2M-1B is a non-magnetic base to hold any of the tube racks.
The dimensions of the base follow the SBS microplate convention so it is ready to use on most robotic workstations as shown in the photo to the right.
The key benefit to this system is the high energy magnet- a V&P specialty!
Mix and match to suit your application! |
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The VP 772F2M-1 is a magnetic base which holds a 48 MGO Neodymium Iron Boron (NdFeB) magnet.
The VP 772F2R-1 is a rack holding FOURTEEN 1.5 ml microcentrifuge tubes.
The VP 772F2M-1B is a non-magnetic base to hold the tube rack.
To order the three parts above use part number VP 772F2M-1kit1
The dimensions of the base follow the SBS microplate convention so it is ready to use on most robotic workstations.
The key benefit to this system is the high energy magnet- a V&P specialty! |
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The VP 772F2M-1 is a magnetic base which holds a 48 MGO Neodymium Iron Boron (NdFeB) magnet.
The VP 772F2R-2 is a rack holding TWELVE 15 ml tubes (16.7mm OD).
The VP 772F2M-1B is a non-magnetic base to hold the tube rack.
To order the three parts above use part number VP 772F2M-1kit2
The dimensions of the base follow the SBS microplate convention so it is ready to use on most robotic workstations.
The key benefit to this system is the high energy magnet- a V&P specialty! |
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The VP 772F2M-1 is a magnetic base which holds a 48 MGO Neodymium Iron Boron (NdFeB) magnet.
The VP 772F2R-3 is a rack holding SIX 50ml tubes (29mm OD).
The VP 772F2M-1B is a non-magnetic base to hold the tube rack.
To order the three parts above use part number VP 772F2M-1kit3
The dimensions of the base follow the SBS microplate convention so it is ready to use on most robotic workstations.
The key benefit to this system is the high energy magnet- a V&P specialty! |
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