Bead/Cell Mixing Reservoirs

     
 


Keeping resins, assay beads, SPA beads, glass beads and cells in suspension while they are being pipetted to microplate wells is very important if reproducible results are to be obtained.  It is also important to do this with the smallest dead volume possible when the reagents are very expensive.  The unique V&P Scientific Bubble Paddle Reservoir mixing systems (US Patent #6,461,034 & European Union Patent #1,314,471) provide a very simple and inexpensive way of accomplishing this goal.  Whether you are using a 4 tip, an 8 tip, a 96 or 384 pipetting head, there is a mixing reservoir for you.

Endorsement

Here is what one of our customers at a major Pharmaceutical company had to say about our 384 Bubble Paddle Reservoir system  "The reservoir is really the best thing that I have found for YSi SPA beads which immediately fall out of solution when not mixed" ..... The bubble paddle is essential for YSi beads." 

 

 
 
 
   

The blue dye in the photo on the right illustrates the contours we have machined into the bottom of the stirrer.  These unique contours coupled with unique Bubble Paddle results in very vigorous stirring of the liquid contents in the reservoir.  The unique feature of the Bubble Paddle Reservoirs is their exclusive design that eliminates "fall out zones" where particulates fall out of solution that are so prevalent with other mixing reservoirs.  Even solutions as viscous as honey (15,000 centistokes) are easily stirred.

 

The VP 756, VP 756A, VP 756A-2, VP 750 and VP 755 Bubble Paddle Reservoirs all must be driven by one of our many Magnetic Alligator Tumble Stirrers.  One of these stirrers can power several Bubble Paddle Reservoirs at the same time. 

The Bubble Paddles are constructed of passivated stainless steel. 

 

The 9 Bubble Paddle reservoirs are constructed from autoclaveable polypropylene.

 

For chemical resistance properties of Polypropylene and other commonly used plastics follow this link.

 

We also have Bubble Paddle Reservoirs to stir magnetic beads.

 

 
 


Download video of Bubble Paddle in action
(mpeg movie- 685KB)


Press the play button to view the video in a flash format, flash must be installed, or click the download link to download a mpeg version.

 
 
 


The Bubble Paddles for the reservoir are made with magnetic stainless steel and have PTFE bearings at the ends which fit into locating bearing notches in the reservoir.  This keeps the Bubble Paddles in precise location in the reservoir and prevents them from touching the bottom of the reservoir.  This latter feature is important when stirring fragile mammalian cells.

The photo to the right demonstrates a VP 581B SBS Adaptor Plate attached to a VP 756 Reservoir. This allows for the VP 756, VP 756A,  to be fitted to any SBS footprint.

 
 
 

 
      Videos: Bubble Paddle Reservoir in action
 

         
 

VP 756A-LID

     
         
 

Polypropylene lid for the VP 756A. It is slotted to allow for pipette tip access.

 

 
 



VP 750-13-AL

     
 


The VP 750-13-AL to the right is made from anodized aluminum and is used to keep the contents warm and suspended.  The aluminum's superior heat conductive properties are very useful in keeping  molten agarose containing cells in a liquid state prior to dispensing to a another cooler media where the agarose will solidify around the cells.  We have placed a brown liquid in the bottom of the reservoir so you can visualize the ducting pattern in the bottom of the reservoir better.

 
 
 

 
  Technical Documents:
    CAD drawing of dimensions
 


VP 750-13-PTFE

     
 


The VP 750-13-PTFE to the right is made from PTFE and was used to create a larger reservoir to keep particulates in suspension while being pipeted by a liquid handling system.  In this case the customer used an eight channel pipetor to dispense the liquid.

 
 
 

The photo to the right illustrates a VP 750-13-PTFE on top of a VP 710C1 Tumble Stirrer.

 

 

Reservoir Specifications:
 

Max. volume: ~80 ml
Dead volume: ~4 ml (when using a 12-channel pipettor, drawing ~300ul per aliquot)

 
 
 

 
  Technical Documents:
    CAD drawing of dimensions
 


VP 750-13-PP

     
 

The VP 750-13-PP Bubble Paddle Reservoir to the right is the same as the VP 750-13-PTFE but made of autoclavable polypropylene. It is used for pipetting with a 12-channel pipettor but can also be used with 8-channel pipetting.


This Reservoir can be stirred with any of our Tumble Stirrers plus the correctly sized reservoir holder accessory.

 
 
 

The photo to the right shows a VP 750-13-PP on top of a VP 710D1 Tumble Stirrer.

 

 

Reservoir Specifications:
 

Max. volume: ~80 ml
Dead volume: ~4 ml (when using a 12-channel pipettor and drawing ~300ul per aliquot)

 
 

 
  Technical Documents:
    CAD drawing of dimensions
 

         
 

Vertical Bubble Paddle Stirrer - VP 754V-15 & VP 754V-50

     
         
 

The Vertical Bubble Paddle stirrer was made to stir magnetic beads and keep them in suspension in a 15 & 50ml tube. Paired with our VP 710D2, it is the perfect solution to keep magnetic beads, cells, and particulates in suspension.

 

Our technology allows us to use a magnetically driven stir element to stir magnetic beads without compromising consistent even mixing.

 

You can use a pipet tip, or tube to obtain your sample while the solution is being stirred.

 

   
         
 

 

Vertical Bubble Paddle Stirrer being used with the
Formulatrix Mantis

 



 
 


VP 750

     
 


The VP 750 Bubble Paddle reservoir is used in conjunction with 96 pipetors on robotic work stations or with 96 pin tools.

 

The center to center distance between the 13 bubbles on the paddle was designed on 9 mm centers so that the pins from 96 pin Replicators or tips from 8 by 12 pipetting systems, will fit in the nodes between the bubbles on the paddle blades.  This spacing allows for continuous stirring while samples are being taken even from the bottom of the reservoir. 

 

Reservoir Specifications:
144 mm long, 91 mm wide, 50 mm tall
Reservoir height = 43 mm**
Reservoir volume = 370 ml
Dead volume = 10 ml


**Custom reservoirs with less height can easily be made to accommodate shorter pipet tips.

 

 
 


VP 710C1-ALPFX

The photo on the right illustrates how we made some simple modifications to a
VP 710C1 and mounted it on its side under one of our FXALP-PM plate holders for the FX and placed the VP 750-PTFE on top.  Follow this link for more information.

 
 
 

 
      Videos: Bubble Paddle Reservoir in action
 

 



VP 756A-2

     
 


The VP 756A-2 with its 2 bubble paddles is used when larger volumes need to be resuspended for pipeting on an 8 strip pipetor.
 

 
 
 

 
      Videos: Bubble Paddle Reservoir in action
 

 



VP 750D & VP 750PTFE

     
 


The 96 Tumble Bubble Paddle Reservoirs are made with Delrin VP 750D or with  PTFE VP 750PTFE.  The Delrin reservoir is not autoclaveable but more economical then the autoclaveable PTFE  reservoir.

 

The Bubble Paddles are constructed of passivated stainless steel. 

 
 
 


Modification of Bubble Paddle Reservoirs to Attach the SBS Footprint

 
 


All of our Bubble Paddle Reservoirs can be mounted on a polypropylene block with SBS microplate dimensions (VP 581B).  Mounting is done by drilling and tapping holes in the bottom of the reservoir.  When ordering your reservoir also order VP 581B-1 and your reservoir will be attached to a VP 581B SBS feature.  This will allow your Bubble Paddle Reservoir to be placed in the portrait position of the following Tumble Stirrers VP 710C-1, VP 710C1-7, VP 710G6, VP 710MXL7T, VP 710MXM7T

 The advantages of the SBS feature on the bottom of the reservoir is two fold: 

1. It will the position the reservoir so a robotic pipettor will be able to place the pipet tips in the correct locations between the bubble paddles. 

 

2.  The reservoir can be taken off the deck and replaced in exactly the same position.

 
 
 


VP 750D-SBS

     
 


The VP 750D-SBS version is designed to be placed on the microplate holding plate used with  VP 710C-1, VP 710C1-7, VP 710G6, and VP 710MXL7T Tumble Stirrers.  The bottom of the reservoir is sculpted to fit into the "Portrait" SBS plate holding feature we have incorporated onto these Tumble Stirrers decks.

 

The advantages of the SBS feature on the bottom of the reservoir is two fold: 

 

1. It will the position the reservoir so a robotic pipettor will be able to place the pipet tips in the correct locations between the bubble paddles. 

 

2.  The reservoir can be taken off the deck and replaced in exactly the same position.

 

Also available in PTFE, as VP 750PTFE-SBS.

 

The photo to the right illustrates the bottom on the reservoir and the SBS    (127.7 mm by 85.5 mm) feature we have machined into the bottom surface.

 

Reservoir Specifications:
144 mm long, 91 mm wide, 50 mm tall
Reservoir height = 43 mm**
Reservoir volume = 370 ml
Dead volume = 10 ml

 

 

 
 


By placing locating features on the bottom of the Tumble Stirrer, it can be located precisely on the deck of many robotic platforms.  The VP 710G6 shown to the right has two locating pins in the bottom plate which register the Stirrer to a PerkinElmer EP3 robot deck.  Consequently this registration will result in the "pick points" in the VP 750D-SBS being positioned precisely under the EP3 pipet tips for that deck position. 

 

Placing a locating feature in the bottom plate of one of our Tumble Stirrers is a custom service we provide.  Just send us a drawing or a deck tile from your robotic platform.

 
 
 

Download Video
(mpeg movie- 4MB)

 

Press the play button to view the video in a flash format, flash must be installed, or click the download link to download a mpeg version.

     
 


The VP 710F Rotary Tumble Stirrers  shown  on the right are small enough to comfortably fit on or under the deck of most robotic workstations and stir the contents of one or more Tumble Bubble Paddle Reservoir.

 
 


VP 750-ECON-6

     
 


The VP 750-ECON-6 96 Tumble  Economy Bubble Paddle Reservoir is used in conjunction with 96, 8 or 12 pipetting heads.  This system uses a Polycarbonate frame inside the VP 792D disposable polypropylene Nalgene reservoir that we also use with our Cybio mixing system.   The whole system is autoclaveable.  The VP 750P-Econ-6 differs from our VP 750D in several respects:

  1. It is smaller and has the same SBS foot print as a microplate (127.7 mm x 85.5 mm).  This allows it to be placed in standard microplate deck tiles without modification.  Just place one of our many VP 710 series Tumble Stirrers under the robot deck and keep cells and beads in suspension while you pipet them into microplates.
     

  2. Each Bubble Paddle is registered in a PTFE bearing inside the Polycarbonate frame instead of the machined complex ducting design used to increase stirring efficiency.  The stirring efficiency in the groove is sufficient for keeping cells and light beads in suspension, but not glass SPA beads. 
     

  3. There are only 11 Bubbles per Paddle.
     

  4. The top of the reservoir accepts a standard microplate lid.

Reservoir Specifications:
127.7mm long, 85.5 mm wide, 50 mm tall
Reservoir height = 40 mm

Reservoir depth = 37 mm
Reservoir volume = 280 ml
Dead volume = 10 ml*

 

* = Depends on aliquot volume,  but with very small aliquots basically all but 10 ml can be recovered.

 

 
 

The VP 750-ECON-6 consists of six components: 1 VP 750P-ECON-5F Polycarbonate-PTFE bearing frame, 4 VP 757AFe-1 stainless steel Bubble Paddles with 11 Bubbles each, and one 280 ml Nalgene disposable polypropylene reservoir.  The later is also sold separately in sterile case lots of 40 as the  VP 792D

 
 
 
 


Download video of Bubble Paddle in action
(mpeg movie- 685KB)


Press the play button to view the video in a flash format, flash must be installed, or click the download link to download a mpeg version.

 
 
 
   
 
 

VP 750-ECON-6A

     
 


The VP 750-ECON-6A 96 Tumble Economy Bubble Paddle Reservoir is used in conjunction with 96, 8 or 12 pipetting heads. This system uses a Polycarbonate frame inside the VP 792D disposable polypropylene Nalgene reservoir. It is similar to the VP 750-ECON-6 except the bubble paddles are positioned perpendicularly so that the reservoir may be placed in the "landscape" position with respect to the Alligator Tumble Stirrer.

 
 
     
 
     
 
         

VP 750AL-2

     
 

The VP 750AL-2 is a Hydrophilic-Coated Aluminum Reservoir. 127.7mm long, 85.5mm wide, 50mm tall. Flat Bottom, designed to hold VP 750P-ECON-5F Polycarbonate Frame with four VP 757AFe Bubble Paddles

 

 
         


VP 750AL

     
 

The VP 750AL version is designed to be placed on a Peltier heater/chiller on a robot deck with a V&P Tumble stirrer on the side of the reservoir.  The reservoir has the same x and y dimensions as a microplate (SBS standard).

 

The advantages of the SBS dimensions of the reservoir is four fold: 

 

1.   The aluminum body conducts the heat or cold efficiently to keep the stirred contents either hot or cold when placed on a Peltier heater/chiller block.

 

2.    It will position the reservoir so a robotic pipettor will be able to place the pipet tips in the correct locations between the bubble paddles. 

 

3  The reservoir can be taken off the deck and replaced in exactly the same position.

 

4.   The reservoir is protected with a Hydrophilic coating.  This Hydrophilic coating also has the advantage of preventing droplet formation and reduces air bubble formation in crevices.

 

 

Reservoir Specifications:
127.7 mm long, 85.5 mm wide, 43 mm tall
Reservoir height = 40 mm**
Reservoir volume = 270 ml
Dead volume = 2 ml***

 

**   = To the bottom of the pipet depressions.

*** = Depends on aliquot volume,  but with very small aliquots basically all but 2ml can be recovered.

 
 
 
 


VP 750AL with VP 540A blotting media and VP 540AC lint free blotting media which can be used to serve as a lid or shield photo sensitive reagents from the light.

 
 
 

 

 
 
 

The photo to the right illustrates the heat exchange fins on the bottom of the reservoir to facilitate the exchange of heat to the Peltier chiller.

 
 
 


 

 
 
 

VP 755AFe-1

     
 


The VP 755AFe-1 Tumble Bubble Paddles are used so that 8 different cell types, or 8 different bead types (in rows) can be re-suspended and pipetted either 8 at a time or 96 at a time into 96 or 384 well microplates. The VP 755AFE-1 Tumble Bubble Paddles are to be used with the VP 572DC (sold separately) SBS disposable polypropylene reservoirs with 8 separate rows. Each bubble paddle goes into each row and will keep your cells, beads, or particles in suspension while pipetting. The whole system is autoclaveable.


The second half of the movie below illustrates how effectively the Bubble Paddles are able to re-suspend 50 micron diameter dense glass beads in the rows of the microplate.

 
 
 


Download video of Bubble Paddle in action
(mpeg movie- 36.5MB)


Press the play button to view the video in a flash format, flash must be installed, or click the download link to download a mpeg version.

 
 
           
 


VP 755D

     
 


The 384 Tumble Bubble Paddle reservoir is used in conjunction with 384 robotic pipetting systems or with 384 pin tools.  The pipet tips or pins remove liquid from the elevated columns between the bubbles while the paddles continue to spin.  The bottom of the reservoir is ducted to conform to the paddles.   The conformation of the Bubble Paddle with the ducting in the reservoir bottom combine to achieve maximum liquid movement and the re-suspension of solids (beads, cells and particulates). 

 

Incorporation of this stirrer into a robotic system to process SPA bead assays was presented at the 2003 MIPTEC meeting in Basil Switzerland.

 
 
   

The 384  Tumble Bubble Paddle Reservoirs are available either in Delrin (not  autoclaveable) VP 755D or in aluminum which is autoclaveable VP 755AL. 

 

Reservoir Specifications:
134 mm long, 86 mm wide

Reservoir height = 32 mm tall
Reservoir depth = 29 mm**
Reservoir volume = 180 ml
Dead volume = 10 ml


**Custom reservoirs with less height can easily be made to accommodate shorter pipet tips.

 
 
         


VP 755D-1

     
 

The VP 755D-1 will replace the VP 755D for most applications.  The difference between the two versions is that the VP 755D-1 has an SBS dimension feature machined into the bottom so that it can simply be placed on the portrait deck positions of our tumble stirrers or the deck positions of many different robot platforms where an Aligator Tumble Stirrer is placed underneath the deck position.

 

 

 
 
 

VP 755D-1 Reservoir Specifications:

134 mm long, 86 mm wide at the top and 85.48 mm wide at the bottom

Reservoir height = 32 mm tall

Reservoir depth = 30 mm**

Reservoir volume = 180 ml

Dead volume = 10 ml

 

**Custom reservoirs with less height can easily be made to accommodate shorter pipet tips.

 
 
         


  Replacement Bubble Paddles

     
 


Replacement Bubble Paddles are available in 7, 9, 11, 13 and 25 bubble versions.  Standard material is passivated stainless steel with PTFE hubs

 

 

Table of Reservoir & Bubble Paddle Compatibility

Reservoir compatibility Format # of Bubbles Coating Part Number
VP 751AL, VP 751P 96-C* 7 None VP 751AFe
VP 756, VP 756A-2 96-C 9 None VP 756AFe
VP 750P-ECON1
VP 750P-ECON2
96-R** 11 None VP 757AFe
VP 750, VP 750-13-AL,
VP 750D, VP 750D-SBS,
VP 750PTFE
96-R 13 None VP 750AFe
VP 755AL, VP 755D 384-R 25 None VP 755AFe

* = Column format
** = Row format

 

 





 
   
Magnetic Bubble Paddle Reservoir for 8 pipet tips, includes 1 Bubble Paddle with 9 bubbles each, holds 25 ml, 96 mm long, 40 mm wide, 24 mm tall, 15 mm deep.  Autoclaveable, made from polypropylene and stainless steel
 
 VP 756
Magnetic Bubble Paddle Reservoir for 8 pipet tips, includes 1 Bubble Paddle with 9 bubbles each,  holds 50 ml, 96 mm long, 40 mm wide, 38 mm tall, 30 mm deep.  Autoclaveable, made from polypropylene and stainless steel
 
 VP 756A
Magnetic double Bubble Paddle Reservoir for 8 or 16 pipet tips, includes 2 Bubble Paddles with 9 bubbles each, holds 75 ml, 96 mm long, 64 mm wide, 38 mm tall, 28 mm deep.  Autoclaveable, made from polypropylene and stainless steel
 
 VP 756A-2
Magnetic Bubble Paddle Reservoir for 12 pipet tips, includes 1 Bubble Paddle with 13 bubbles each,  holds 70 ml, 143 mm long, 40 mm wide, 38 mm tall, 30 mm deep.  Autoclaveable, made from anodized aluminum and stainless steel
 
 VP 750-13-AL
Magnetic Bubble Paddle Reservoir for 96 pipet tips, includes 4 VP 750A Bubble Paddles with 13 Bubbles each, holds  180 ml, 143 mm long, 90 mm wide, 49 mm tall,  43 mm deep.  Not Autoclaveable, made from Delrin and stainless steel
 
 VP 750D
Magnetic Bubble Paddle Reservoir for 96 pipet tips, includes 4 VP 750A Bubble Paddles with 13 Bubbles each, holds  180 ml, 143 mm long, 90 mm wide, 49 mm tall,  43 mm deep.  Autoclaveable, made from PTFE and stainless steel
 
 VP 750PTFE
Magnetic Bubble Paddle Reservoir for 384 pipet tips,  includes 8 VP 755AFeP Bubble Paddles with 25 Bubbles each, holds 160 ml, 140 mm long, 86 mm wide, 26 mm tall,  23 mm deep.  Not Autoclaveable, made from Delrin and stainless steel
 
 VP 755D
Magnetic Bubble Paddle Reservoir for 384 pipet tips,  includes 8 VP 755AFeP Bubble Paddles with 25 Bubbles each, holds 160 ml, 140 mm long, 86 mm wide, 26 mm tall,  23 mm deep.  Autoclaveable, made from Aluminum and stainless steel
 
 VP 755AL


REPLACEMENT BUBBLE PADDLES

Bubble Paddle, 13 bubbles on 9 mm centers, constructed from stainless steel and PTFE hub.  VP 750AFe
Bubble Paddle, 25 bubbles on 4.5 mm centers, constructed from stainless steel and PTFE hub.  VP 755AFe
Bubble Paddle, 9 bubbles on 9 mm centers, constructed from stainless steel and PTFE hub.  VP 756AFe
 
         

About | Ordering | Returns
Copyright 2010, V&P Scientific, Inc. All rights reserved.