Fu-Meei Registration System

     
         
 

The Fu-Meei Registration System provides an efficient way of making identical DNA blots on multiple small membranes (xeroxing).  It has its own built in three pin spindle system (VP 440) for registering the Replicator to the membrane that also registers the blots to locating (reference/spindle) holes punched in the membrane.

The locating hole features allows image analysis equipment convenient reference features to locate and identify positive blots.  Furthermore our custom paper punch (VP 441) is a convenient way to punch holes in many nylon membranes (VP 502) simultaneously and place a hundred or more of them on the three pin spindle for blotting.  The spacing of the hole pattern also is used to orient the user to location of the blots, right from left and top from bottom.

The MULTI-PRINT™ (VP 382F) is placed on top of the three pin spindle (VP 440) and any MULTI-BLOT™ Replicator may be used to make blots on the membranes.  Once the blot is made, simply remove the MULTI-PRINT™, top membrane and separation paper, replace the MULTI-PRINT™ and repeat the blotting process.  It is as simple as 1, 2, 3.

Depending upon the application, you can make blots from 0.1 ul to 10.0 ul with our MULTI-BLOT™ Replicators.  We recommend the 1 ul 384 Slot Pin MULTI-BLOT™ Replicator (VP 384S) and nylon membranes (VP 502) for maximum blot size and resolution.  When using 96 well plates you may find it desirable to use larger volume Slot Pin Replicators.

The Fu-Meei Registration System may also be used to make high density arrays by using MULTI-PRINTS that have a 2 by 2 array alignment pattern (VP 382F1) for 384 Pin MULTI-BLOT™ Replicators, a 3 by 3 array alignment pattern (VP 382F2), and 4 by 4 array alignment pattern (VP 382F3) for 96 Pin MULTI-BLOT™ Replicators.  When making high density arrays we recommend that you use the VP 409 MULTI BLOT™ Replicator or VP 386 MULTI-BLOT™ Replicator.

If you are transferring DNA, cDNA or other materials in hydrophobic solutions you will find that using our new V&P Pin Cleaning Solution will greatly increase the precision of delivery.  For an explanation see the FAQ page and the V&P Pin Cleaning Solution page.


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MULTI-PRINT™ for Fu-Meei Registration System, one alignment position (384) VP 382F
MULTI-PRINT™ for Fu-Meei Registration System, 2 x 2 alignment array (96) VP 382F1
MULTI-PRINT™ for Fu-Meei Registration System, 3 x 3 alignment array (96) VP 382F2
MULTI-PRINT™ for Fu-Meei Registration System, 4 x 4 alignment array (96) VP 382F3
1 ul 384 Slot Pin MULTI-BLOT™ Replicator VP 384S
1 ul 96 Slot Pin MULTI-BLOT™ Replicator VP 408S
Fu-Meei 3 pin Registration Spindle Platform VP 440
Fu-Meei Registration Membrane Punch VP 441
Hybond N Nylon Membranes, 10 cm x 15 cm VP 502
 
         
 

Fu-Meei Registration Starter Kit VP OK11

MULTI-PRINT™ for Fu-Meei Registration System, one alignment position (384) VP 382F
1 ul 384 Slot Pin MULTI-BLOT™ Replicator VP 384S
Fu-Meei 3 pin Registration Spindle Platform VP 440
Fu-Meei Registration Membrane Punch VP 441
Hybond N Nylon Membranes, 10 cm x 15 cm VP 502
One, Bottle of pin cleaning solution VP 110
One, Package of lint free blotting media VP 522
 


  Accessories

     
 

We suggest that you buy 5 other inexpensive items when you purchase a Replicator that will make using the Replicators easier, safer and more reproducible.  The VP 381 is a Registration Device that aligns the Replicator pins to the center of the wells.  This is very important as you do not want the pins to touch the side of wells as you remove the pins because that will knock off the hanging drop at the pin tip.  The VP 430 is a Reading Mat that helps locate which mother plate well produced a positive spot on a membrane array.  The VP 420 is a Glass Alcohol Reservoir that is used in the sterilization of the Replicators by flaming.  Some labs use plastic reservoirs but if flaming alcohol drops into them the plastic burns too dumping flaming alcohol all over the counter.  The VP 425 is Pin Cleaning Brush that is used to kept the Replicator pins clean so they will carry the same amount of liquid each time.  VP 110 is a Pin Cleaning Solution that is used to clean and treat the pin tips when they can't be flamed.

 
 


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