Robot Pin Tool Systems

     
 

Compatible Robots
Pin Tool Data
Docking Stations
Storage Stations
Tip Loader Adaptors
Robot Mounts
Fixture Frames
Pin Delivery Volumes
Pin Tool Cleaning

Defining a Pin Tool
Pin Tool Weights
Pin Tool Heights
Pin Tool Accessories
Assay protocols
Pin Tool Brochure
Tecan Pin Tool Overview
Robotic's Companies that distribute our Pin Tools
 
 


Versatility

     
 
As the trend towards assay miniaturization continues with increasing demands to make nanoliter transfers, here is a simple way to convert either a 96 or a 384 Well Pipetting System or plate handler into a pin tool work station that will deliver from 2 nl to 5 ul using 96, 384 or 1,536 pin tools.   We have developed 2 basic floating pin tool styles (E-clip and Tube type) and now can mount them to a variety of robot pipetting systems and microplate handling systems.  Our standard mounting plates allow all our pin tools to be easily attached to 36 other Robot pipetting systems or to microplate handling systems.  We have also made many custom mounting plates.  For example Harvard Medical School uses our Pin Tools on a Seiko robot.
 
 


***NEW***

     
 

Our mounting plates for the MWG, Beckman FX Gripper and the Tecan Roma Arm robot plate handlers may be compatible with many other robot plate handlers.  Depending upon how your plate handler picks up the plate it may be able to interface with one of these 3 plate handler mounting systems.  If that is the case you can convert your plate handler into a pin tool robot.  Just have your plate handler pick up the pin tool from a VP 550A Docking Station and program it to perform the simple pin tool assay protocols.

Whether you use the dispense head or the plate handler, to order a pin tool just specify your robot system, application, the number of pins (96, 384, 1536 or other) required, the volume you wish to transfer, the length of the pin, magnetic characteristics, fixed or floating and whether you need a hydrophobic/lipophobic coating on the pin.  We will take that information and give you a quote on an appropriate pin tool that will be compatible with your application and robot system.

 
 


Save Money

     
 

If you have an obsolete 96 or 384 pipeting system or one with damaged pistons you can bring it back on line as a 96, 384 or 1536 pin tool work station.  Not only will you save a ton of money by rehabilitating an obsolete or broken work station,  you will also pay for the pin tool in your first screening operation by eliminating the cost of disposable pipet tips.  At $.12/tip, 200 cases of 384 tips are ~$9,000.00.  Our robot mounted pin tools start at only $1,190.00.

 


  Choose a Standard Mounting Plate From the Following Robot Systems

 
 

Apogent Discoveries
      (Matrix PlateMate 96)
 
Genomic Solutions
      GeneMachines G3
Qiagen 8000

Apogent Discoveries
      (Matrix PlateMate 384)
 

The Genomics Institute of the Novartis Research Foundation (GNF)
 

Robbins Hydra
 

Apogent Discoveries
      (Matrix PlateMate Plus)
 

Hamamatsu

Sagian MultiPette

Apricot Designs
 

Hamilton Microlab 96/48
 

Seiko
 

Apricot Designs Model N
 

Hamilton Microlab Star

Tecan RoMa Arm

Beckman BioMek
 

High Resolution Engineering Micropin

Tecan Te-MO

Beckman FX

Labcyte DeckPette

TekCel Assay TekBench

Beckman FX Gripper
 

Labcyte DynaPette
 

The Automation Partnership
 

Beckman FX Reset
 

Molecular Devices FLIPR

Tomtec 96SV

Beckman MultiMek
 

Molecular Devices FLIPR Tetra

Tomtec 384
Beckman Magnetic Mounting
MWG 4204SE
Tomtec Quadra

Caliper (Zymark) Sciclone
 

Novartis
Agilent (Velocity11)

Cybi Well 96
 

PerkinElmer EP3
      (CCS Packard)
 
 

Cybi Well 384
 

PerkinElmer MiniTrak
      (CCS Packard)
 
 

Dynamic Devices
 

PerkinElmer PlateTrak
      (CCS Packard)
 
 

Genetix Qpix
 

Qiagen 3000
 
 

 


Robotic's Companies that distribute our Pin Tools

     



Apogent Discoveries (Matrix) Platemate Plus Pin Tools

 
 


The PlateMate Plus pin tool on the right illustrates how we use a "bridge plate" to couple a V&P pin tool to the disposable tip carrier.  This is a simple and inexpensive way of adapting a V&P pin tool.

 

 
 


Although very similar to the CyBio and PlateMate robot platforms the PlateMate Plus can accommodate the taller FP_, FP_N and FP_T pins, illustrated in the photo to right using FP1 pins.

 
 
 


Apricot Designs

     
 


The Apricot pin tool to the right illustrates a 96 format pin tool with FP floating pins.

 
 
 


Beckman FX Pin Tools

     
 
The VP 384FP1CB Pin tool on the right was mounted onto the Beckman FX standard pin tool feature.  If you already have an Beckman FX pin tool this is a very easy modification for us to make.  We can even use the Beckman FX to reseat the pins in the rare event that they get stuck in the up position.  If you don't already have an FX with their pin tool accessory, we can also provide another solution.

To use our pin tools on the Beckman FX dispense head you will also need to purchase from Beckman their Tool Body Kit p/n719894 which contains a CD with an HDR patch that will convert FX software from v2.1 to v2.2 so the FX can be programmed to accept the different V&P pin lengths.  The Gripper Tool still functions with the V&P Pin Tool attached allowing  "on the fly" work surface changes.

 
 
 

 
Beckman FX Gripper Pin Tool

     
 


The floating pin tool on the right has a Beckman FX Gripper mounting  with indentations that allow the Gripper to easily pick it up and manipulate it in an assay sequence and then park it in a docking station while it performs other functions.  This ability would allow the FX to manipulate several pin tools at the same time and perform complex nanoliter dispensing operations.

 

 


Beckman Magnetic Mounting Pin Tool

     
 


Beckman makes a magnetic mounting plate to mate to all V&P pin tools. This feature facilitates "pin tool on the fly" operations.  Just park the pin tool in a docking station while you perform pipetting operations.  Contact Beckman directly for the purchase of this mounting plate.

 
 
 


Carl Creative Systems Hook Mounted Pin Tools

     
 
The VP 384FP1CB Pin tool on the right was mounted onto the Beckman/Sagian hook style plate which can be attached to a MultiMek or Multipette pipeting system.   If you have an obsolete 96 MultiMek or Multipette, you can bring it back on line as a 384 or 1536 nano liter dispensing tool.   The Carl Creative Systems hook mount allows for easy attachment of the pin tool to the robot head.  It attaches just like the tip loading feature. 

The hook mount tip loading feature was made by Carl Creative Systems and sold on an OEM basis to several robotics companies.  The hook mount is also used on the Molecular Devices FLIPR, PerkinElmer (CCS Packard) PlateTraks, MiniTraks and EP3.  If you have this feature on your robot, chances are you can use our Hook Mount to mount a Pin tool on your Robot.  For very precise rotational positioning needed with 1536 applications we suggest you use our hook mount with a rotational adjustment feature.

 

 
 


Dynamic Devices Oasis Pin Tools

     
 


The pin tool is directly attached to the robot and is designed for rapid HTS applications.

 
 
 


Genetix QPIXS Pin Tools

     
 


The BMPGENETIXQ mount allows us to adapt all our V&P pin tools to the Genetix's QPixs.  We have hollowed out this mount so we can use even the longest of our pins.

 

 
 


Hamilton Pin Tools

     
 


The mounting plate on the right was built by Hamiliton for attaching one of our pin tools to the 96 pipetting head of a MicroLab Star Workstation.   The advantage of this system is that the pin tool can be picked up "on the fly" from a docking station, used in a pin tool transfer and then returned to the docking station robotically with out human intervention.   Contact Hamilton about the purchase of the Star Mounting plate.

 
 
 


The mounting plate on the right was built by Hamiliton for attaching one of our pin tools to the 384 pipetting head of a MicroLab Star Workstation. Contact Hamilton about the purchase of the Star Mounting plate.

 
 
 


Pin Tool Docking station on Hamilton deck.

 
 
 


Labcyte DYNAPETTE & DECKPETTE Pin Tools

     
 


The E-Clip style floating pin tool at the right has a mounting plate that mates to either the DynaPette or DeckPette workstations used in liquid handling.   The pin tool in the photo is used to inoculate yeast cells onto to agar plates (Omni Trays VP 413).

 

 
 
 


MWG Pin Tools

     
 


The E-Clip style floating pin tool on the right has a MWG mounting plate with indentations that allow the plate handler to easily pick it up and manipulate it in an assay sequence and then park it in a docking station while it performs other functions.  This "Pin Tool on the Fly" ability transforms a simple plate handling robot to also perform complex nanoliter dispensing operations.  Watch the video below which demonstrates the "Pin Tool on the Fly".

 

 

 

 

 

The image to the right illustrates the notch in the top mounting plate that allows the robot plate handler to securely pick up the pin tool by accommodating the handler gripper to slide under the mounting plate.  This simple design can also be used by other plate handling robot systems.


Download video of MWG Mount in action
(mpeg movie- 3.8MB)

Press the play button to view the video in a flash format, flash must be installed, or click the download link to download a mpeg version.

 

 
 


QIAGEN Pin Tools

     
 


The E-Clip style floating pin tool at the right has a mounting plate that mates to a standard Qiagen accessory used in liquid handling.  The advantage of this mounting system is that the robot lab hand can pick the pin tool up and manipulate it through an assay sequence and then park it in a docking station while it performs other functions.

Click here to view an article where our Qiagen pin tool was cited as being used in yeast transfers.

 
 
 


Robbins Hydra Pin Tools

     
 


The 384 floating pin tool at the right has a mounting plate that mates to the dispense mechanism of the Hydra 96.  The Pin Tool height can be adjusted by adjusting the dispense function.  The bottom platform is raised to dip the pins into the microplates or wash reservoirs.

 
 
 


Seiko Pin Tools

     
 


The BMPSEIKO mount allows us to adapt all our V&P pin tools to the Seiko Robot.  The photo to the right is a fixed 384 pin tool and is courtesy of Jim Follen at Harvard University.

 
 
 


One of our Biotek customers who prefers to remain anonymous sent us a photo of one of our pin tools with a custom mount to a Seiko Robot. They didn’t want the pins to float so they put a foam pad we supply on top of the pins to keep them from floating. They have used our pins both fixed and floating for many years and are very pleased with them.

 
 
 


Tecan RoMa Arm Pin Tools

     
 


The 384 pin tool on the right illustrates a mounting system that has been modified so a RoMa Arm on either a Tecan Genesis or Freedom Workstation can park and pick up the pin tool "on the fly".  This feature allows the pin tool to be "Hot Swapable" with other functions of the RoMa Arm   thus giving the user greater capability in applications. 

 
 


Tecan Te-MO Pin Tools

     
 


The 384 pin tool on the right illustrates how the BMPTECAN Te-MO mounting plate attaches to the pin tool through an intermediate bridge plate.  The BMPTECAN allows the robot to pickup and drop off the pin tool on the fly.  See the movie at this link.

 
 
 


Tecan Rotational Mount

     
 


Basic mounting plate for Tecan TeMO 96 with rotational adjustment feature for use with 1536 pin tools. 
When customers use a 1536 pin tool they need to be able to make very fine rotational adjustments so the pins are placed in the center of the wells.   This placement is essential when using on 1,536 well plates.   We also recommend using it on the 384 pin tools when positional accuracy is necessary.  The BMPTECANR allows you to do that.

 
 
 


Tekcel Assay Tekbench Pin Tools

     
 


The 384 pin tool on the right illustrates a BMPN mounting system that has been modified so the robot can park and pick up the pin tool "on the fly".  This feature allows the pin tool to be "Hot Swapable" with tips and other accessories on the Assay TekBench thus giving the user greater capability in applications.

 
 
 


The Automation Partnership Pin Tools

     
 


The pin tool at the right started out as a custom, we now offer the mounting plates as a standard product.

 

 

 

 

 

 

 

A new addition to The Automation Partnership pin tools is the incorporation of a docking feature on the bottom float plate.  This docking feature mates to a standard docking tool on the robot platform and allows the pin tool to be picked up "on the fly".  The AFIX384FP1TAP has a larger foot print and a thicker bottom float plate than our standard bottom float plates.

 

 

Agilent (Velocity11)
 
 


The Agilent (Velocity11) 96 pin tool on the right illustrates the mounting system we use on the Bravo Workstation.  This system mounts directly onto the Bravo.  The system also has a rotational adjustable feature built into it so it can be used with 1536 pin tools.

 

 
 
Capiler (Zymark) Sciclone "Hot Swapable" Pin Tools
 
 


The Caliper (Zymark) Sciclone 1,536 pin tool on the right illustrates a mounting system that mimics the Caliper (Zymark) tip cartridges.  This feature allows the pin to be "Hot Swapable" with tips and other accessories on the Sciclone thus giving the user greater capability in applications.

 

 
 

In addition to these 34 Robotic Systems we have also mounted our pin tools to many custom robotic platforms.

     


  Typical Robot Installation

     
 


The photo on the right illustrates a Sagian MultiPette outfitted with a 384 pin tool being feed by 2 CCS Packard stackers.  The MultiPette's tip loader station and the wash station have been replaced with VP 540M adaptors and  VP 540 pin wash reservoirs. One reservoir contains DMSO and one hidden from view contains Isopropanol.  Two pin blotting stations (VP 540 DB) are in the foreground.  A movie of the Pin Tool in operation can be viewed below. 

The photo and movie are courtesy of John Herich, Maxim Pharmaceuticals.



Download Video of 384 Pin Tool on a Maxim Robot
(mpeg movie- 6.2MB)

 

Press the play button to view the video in a flash format, flash must be installed, or click the download link to download a mpeg version.

 

 

 
 
The photo to the right and video below illustrates a typical setup and run on an Apricot Designs TPS-384 Robot.

Download Video
(mpeg movie- 16.5MB)


The video below illustrates a typical setup and run on an Apricot Personal Pin Tool Robot.

Download Video
(mpeg movie- 8.7MB)

 

Press the play button to view the video in a flash format, flash must be installed, or click the download link to download a mpeg version.

 
 


  For comprehensive data on nanoliter dispensing with pin tools see the following pages.

 
 
  • DMSO and Aqueous delivery with solid and slot pins
  • Maxim Pharmaceuticals, results with slot pin tools
  • New England LRIG presentation 6/18/02
  • Pin Tool Data Page
     
 

Storage Stations for Pin Tools offer a safe place to store pin tools and keep them clean.

     


  Factors Affecting Pin Delivery Volumes

     
 


The list on below summarizes the factors that contribute to the volume delivered.  With each application these factors can be controlled and standardized so that the delivery volumes are very reproducible.  With most applications the CV's are less than 5%.

 

1. Pin Diameter
2. Surface tension of the liquid being transferred
3. Surface tension of the pin
4. Speed of removal of pin from source liquid
5. Speed of pin striking recipient dry plate
6. Depth to which the pin is submerged in source plate
7. Depth to which the pin is submerged in recipient plate
8. Volume of slot in pin
9. Surface tension of the dry plate and dwell time

     
 
V&P pins transfer liquid by carrying the liquid on the sides, tip and in slots we cut into the tip. The amount delivered is determined by the diameter and surface tension of the pin, the surface tension of the liquid, how far the pin is submerged into the liquid and the speed with which the pin is removed from the liquid.  When all of these variables are fixed, the accuracy of the liquid transfer is very consistent, with CV's averages better than 5%.

Increasing the speed of withdrawal from the source plate by 7- fold will increase the volume delivered by as much as 3 fold in a linear relationship.  This phenomenon can be exploited to expand the range of delivery volume for a single pin. The table and the chart below illustrates the effects of increasing the speed 7- fold.  The speed range is from 0.78 cm/sec to 5.7 cm/sec.  Use this chart to select the pin that will deliver in your desired range.

The Standard Tube style pin tools are easily adapted to be mounted on many robot work stations.  We have made custom mounts for  custom robots and our customers have mounted our pin tools on the Beckman FX and the TekCel work stations.  We now have our own mounting system for the FX.  If you want to use it with a robotic work station just fax us a drawing of the mating feature on your robot and we will incorporate that design into the mating plate.  All of our Standard Tube style pin tools have the mounting features for the Beckman BioMek 2000 already built in.  If you want to use it by hand, please add the BGP to your order.

 
 


Save Money

     
 


If you have an obsolete 96 or 384 pipetting station or one with damaged pistons, you can bring it back on line as a 96, 384 or 1536 pin tool work station.  Not only will you save a ton of money rehabilitating an obsolete or broken work station,  you will also pay for the pin tool in your second screening operation by eliminating the cost of disposable pipet tips.  At $.12/tip, 200 cases of 384 tips are ~$9,000.00.

Docking Stations for Pin Tools provide a safe facility for tool changing robots to park and pick up pin tools.

     
 
V&P pins transfer liquid by carrying the liquid on the sides, tips and in slots we cut into the tips. The amount delivered is determined by the diameter and surface tension of the pin, the surface tension of the liquid, how far the pin is submerged into the liquid and the speed with which the pin is removed from the liquid.  When all of these variables are fixed, the accuracy of the liquid transfer is very consistent, with CV averages better than 5%.

Increasing the speed of withdrawal from the source plate by 7- fold will increase the volume delivered by as much as 3 fold in a linear relationship.  This phenomenon can be exploited to expand the range of delivery volume for a single pin. The table and the chart below illustrates the effects of increasing the speed 7- fold.  The speed range is from 0.78 cm/sec to 5.7 cm/sec.  Use this chart to select the pin that will deliver in your desired range.

 
 

 
  Select The Volume You Need To Transfer For Your Application

 
 
DMSO Liquid To Liquid Transfers With Uncoated Pins
     
 
Pin Diameter Shape Delivery Volume Range
FP9 0.229 mm Solid 5 - 15 nl
FP8 0.350 mm Solid 12 – 28 nl
FP1 0.457 mm Solid 19 –  68 nl
FP1S6 0.457 mm 6 nl Slot 26 – 74 nl
FP1S10 0.457 mm 10 nl slot 31 – 79 nl
FP1S20 0.457 mm 20 nl slot 43 – 89 nl
FP1S30 0.457 mm 30 nl slot 53 – 99 nl
FP1S40 0.457 mm 40 nl slot 64 – 109 nl
FP1S50 0.457 mm 50 nl slot 74 – 117 nl
FP3 0.787 mm Solid 84 – 240 nl
FP4 0.910 mm Solid 112 – 207 nl
FP 1.580 mm Solid Pointed 157 – 595 nl
FP3S100 0.787 mm 100 nl slot 178 – 326 nl
FP2S200 0.787 mm 200 nl slot 278 – 424 nl
FP6 1.580 mm Solid Flat 363 – 884 nl
FP3S500 0.787 mm 500 nl slot 527 – 656 nl
FPS.5 1.580 mm 0.5 ul slot 443 – 906 nl
FP6S.5 1.580 mm 0.5 ul slot 726 – 1,253 nl
FPS 1.580 mm 1 ul slot 938 – 1,424 nl
FP6S 1.580 mm 1 ul slot 1,239 – 1,786 nl
FPS2 1.580 mm 2 ul slot 1,817 – 2,248 nl
FP6S2 1.580 mm 2 ul slot 2,102 – 2,576 nl
FPS5 1.580 mm 5 ul slot 4,475 – 4,828 nl
FP6S5 1.580 mm 5 ul slot 4,475 – 4,828 nl
 
 


*Delivery volume range is determined by speed of withdrawal from source liquid.
slow speed  = 0.78 cm/sec = low volume delivery range
fast speed  =  5.70 cm/sec = high volume delivery range
Follow this link to assay methods

*Speed of withdrawal determines volume transferred. The faster the withdrawal speed the larger the volume transferred.  Withdrawal speed range is from 1.5 mm/sec to 30mm/sec.

 

*The volume of liquid in the source well also makes a significant difference in the volume transferred and there is a difference between sold and slotted pins.  The volume used for the 96 well data was 200 ul and the volume used for the 384 well data was 75 ul.  If you use more or less volume in your wells it will affect the volume transferred.  See this link for more information.

 

The links below are to delivery volume range tables for uncoated and hydrophobic coated delivering either DMSO or Aqueous solutions in liquid to liquid transfers.

DMSO TRANSFERS WITH HYDROPHOBIC COATED PINS

DMSO TRANSFERS WITH UNCOATED (STAINLESS STEEL) PINS

AQUEOUS TRANSFERS WITH HYDROPHOBIC COATED PINS

AQUEOUS TRANSFERS WITH UNCOATED (STAINLESS STEEL) PINS

 

 


  A Guide For Selecting The Right Pin For Your Robot Application

 
 


1.  The first consideration is the type of robot system you want to mate the pin tool to.  We have standard mounting systems for the following robot systems:

Robotic's Companies that distribute our Pin Tools

 
 

Apogent Discoveries (Matrix PlateMate 96)

Apogent Discoveries (Matrix PlateMate 384)

Apogent Discoveries (Matrix PlateMate Plus)

Apricot Designs, (PerkinElmer)

Apricot Designs Model N

Beckman BioMek

Beckman FX

Beckman FX Gripper

Beckman FX Reset

Beckman MultiMek

Caliper (Zymark) Sciclone

Cybi Well 96

Cybi Well 384

Dynamic Devices

Genetix Qpix

Genomic Solutions
GeneMachines G3

Hamamatsu

Hamilton Microlab 96/48

Hamilton Microlab Star

High Resolution Engineering Micropin

Labcyte DeckPette


Labcyte DynaPette

Molecular Devices FLIPR

Molecular Devices FLIPR Tetra

MWG 4204SE

Novartis

PerkinElmer EP3
(CCS Packard)

PerkinElmer MiniTrak
(CCS Packard)

PerkinElmer PlateTrak
(CCS Packard)

Qiagen 3000

Qiagen 8000

Robbins Hydra

Sagian MultiPette

Seiko

Tecan RoMa Arm

Tecan Te-MO

TekCel Assay TekBench

The Automation Partnership

Tomtec 96SV

Tomtec 384

Tomtec Quadra

 

 
 


2.  What volume do you need to  transfer?   DMSO? Aqueous? See these hyperlinked tables to determine your options.  Also consider custom slot pins.

3. Will the source plate have wells with significantly different levels of liquid?  (Cherry picked source plate or edge drying effect?).  If yes and if the absolute volume transferred is critical, then select the largest slot pin that is in your transfer range.  This will minimize the effect of liquid height on the volume of liquid carried on the sides of the pin.  Also consider custom slot pins.

4. Does the material transferred bind non-specifically to stainless steel?  If yes then select the Hydrophobic/lipophobic coated pins.  If no, select the uncoated pins.

5. What is the Z clearance on the robot deck from the highest impediment (top of the source plate/recipient plate/wash reservoirs, etc.) to the top of the robot mounting plate?  If it is greater than 77 mm you can use any of pins.  If it is less than 77 mm, you can only use the shorter FP(#)C series pins (12 mm exposed pin length), the FP(#N) series pins (17 mm exposed pin length) and the E-clip series pins (23 mm exposed pin length pins.  If the Z clearance is less than 60 mm, we recommend you contact us.  There are a few tricks we can do to shave off 5 to 10 mm in height. 

Links to the specifications of all the robot mounting plates/pin tool/pin combinations can be found on the height ("Z") restriction page

6.  Is the source plate a deep well plate?  If so you may need to use a 30 mm long exposed length pin ("T" pin) to reach down to the lower levels of the well. 

7.  Should you choose the solid pin versus the slot pin? Although there appears to be a slight advantage for slot pin, CV's between the two pins are very good.  Both are easy to clean between specimens.  Biggest factor is cost.  If you don't need to deal with varying liquid heights and you can obtain the volume necessary with a solid pin, choose the economical solid pin.

8.    If you are still uncertain about which pin to select for your application, you can perform a simple "Proof of Principle" test with several different pins using our inexpensive VP 450FP3 Replicator Strip coupled via a VP 452MP to one of our robot mounting plates.   An even simpler solution is to use a work station with a 1, 4 or 8 probe dispense head with the new V&P Mono Pin Tool to test the various pins.

 


  Liquid To Dry Plate Transfers For Multiplexing Assays

     
 


Many of our customers want to make liquid to dry plate transfers to make mini-microarrays in a single well for multiplexing assays.  By their very nature these transfers only leave a small volume remaining on the plate.  The amount remaining depends on the surface tension of the plate, the transfer liquid and the pin (diameter and shape).  We have found that reasonable results can be obtained if you use an aqueous spotting buffer and a polypropylene surface (CV's less than 9%).  DMSO spotting buffers leave much less liquid on the dry plate and when used with polystyrene plates the CV's are significantly higher. 

The plate to the right was spotted using a 50 nl slot pin (FP1CS50) transferring oligo DNA in an aqueous spotting buffer to a specially treated polypropylene plate.  Our customer in this case wanted to place 42 individual spots in the bottom of each well of a 96 well microplate.  Spot diameter = 0.55 mm. Volume transferred to the spot = 5.85 nl

The photo on the right is the result of a four fold serial dilution hybridization assay of the Oligo spots with a labeled DNA that can be detected optically and quantitated.

Follow this link for more information on liquid to dry transfers and Multiplexing.

 

 

 

 
  Defining a Pin Tool

     
 


Originally we sold our pin tools with unique part numbers.  As we have added more pins to our selection and more robot systems to mount the pin tools to, the number of unique part numbers grew exponentially to an un-manageable size.  Our solution has been to specify a pin tool by the 3 component parts that compromise the Pin Tool.  The Pins, the Floating Fixture and the Robot Mounting Plate.  If you have previously ordered a pin tool by unique part number, we will be able to provide the same pin tool to you.

   

 

 



 


  Selecting a Pin Tool as Simple as 1, 2, 3

   
 


1. Select the appropriate pin. 
For the manual and Beckman Bio Mek 2000 robots we recommend the longer FP_ series pins.

2. Select the Floating Fixture with the appropriate pattern (96, 384 or 1,536) and diameter of holes.

3. Select the robot system you wish to use the Pin Tool with.  We have made Mounting Plates to over 34 Robot Workstations.  The BMP Mounting Plate on this page accommodate the manual pin tool and the Beckman BioMek 2000 robot.

Determine the weight of a Robot pin tool at this link

 

   


  Convert a Robot Pin Tool to a Manual Pin Tool

     
 

 

Our Robotic floating Pin Tools can easily be converted to a manual pin tool with this simple BGPK  kit which contains a Guide Pin Frame and handle and sturdy mounting plate (BMPM)  The guide pins are used with our manual microplate (VP 381N) and membrane registration accessories (VP 382 and VP 382B) to keep the pins in the center of the wells and to make high density arrays on membranes or agar surfaces

Conversion of robot pin tools to manual Multi-Blot Replicators is very easy using our BGPK for Standard length pins and the BGPKL for longer pins.  Simply remove the robot mounting feature from the float plates disassemble the pin tool and reassemble it with the Guide Pin Frame as part of the tool.  Attach the Manual Mounting Plate and handle and you are ready to go.   If this sounds too daunting for you - send it back to us and we will do it for you for a nominal fee.

The main reason for this conversion is to attach  guide pins to the replicator so it can be aligned to Microplate registration accessories such as the VP 381 series Library copiers for replicating and reformatting genomic libraries, VP 380 series colony copiers to make high density arrays on agar surfaces or  VP 382 membrane printers to make high density arrays on nylon or other membranes.

The BGPKL kit is used when longer replicator pins are used like the FPAM pins which are 63 mm long.   A pin tool with FPAM pins is illustrated in the photo to the right.   In order to accommodate these longer pins we need to use a longer and more sturdy guide pin found on the BGPL frame.  These larger guide pins are 3.17 mm in diameter.

Each of the kits on the right (BGPKL and BGPK) is made up of a handle, a manual mounting plate and a guide pin frame.  The guide pin frame is the only  difference between the two kits

 

 
 


For comprehensive data on nanoliter dispensing
with pin tools see the following pages.

 
       
         

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